Biology II - Syllabus

Embark on a profound academic exploration as you delve into the Biology II course () within the distinguished Tribhuvan university's CSIT department. Aligned with the 2065 Syllabus, this course (BIO-157) seamlessly merges theoretical frameworks with practical sessions, ensuring a comprehensive understanding of the subject. Rigorous assessment based on a 60+20+20 marks system, coupled with a challenging passing threshold of , propels students to strive for excellence, fostering a deeper grasp of the course content.

This 3 credit-hour journey unfolds as a holistic learning experience, bridging theory and application. Beyond theoretical comprehension, students actively engage in practical sessions, acquiring valuable skills for real-world scenarios. Immerse yourself in this well-structured course, where each element, from the course description to interactive sessions, is meticulously crafted to shape a well-rounded and insightful academic experience.

Course Synopsis: Cell Division, DNA structure and function, RNA, transcription and translation process, mutation, gene regulation, recombinant DNA technology.
Goal: The course is aimed at knowing the living organism at the molecular level. It also focused on techniques for gene manipulation by using recombinant DNA technology.


Unit 1

Cell division: Mitosis, meiosis, mechanism of crossing over, non-disjunction, ell cycle, abnormal cell division, basis of oncology

Unit 2

DNA: Structure of DNA, replication of DNA, Organization of DNA in chromosomes, forms of DNA

Unit 3

3.1    RNA: Overview of gene expression, transcription-synthesis of RNA, process, structure of mRNA

3.2    Protein synthesis: Decoding the message, tRNA, ribosomal rNA, role of ribosome in protein synthesis

3.3    Genetic code: Introduction of genetic code, wooble hypothesis

Unit 4

Mutation and DNA repair: Introduction, types of mutation, reversion, mechanism of DNA repair.

Unit 5

5.1 Gene regulation in prokaryotes: Operon concept, transcriptional control of protein synthesis, post transcriptional gene control

5.2 Eukaryotic gene control: Control of transcription, post transcriptional gene control, splicing.

Unit 6

Recombinant DNA technology; introduction, tools for cloning, vectors and restriction endonucleases, gene cloning and expression, application of recombinant DNA in healthcare and agriculture industry